StepOnePlus™ - Real-Time PCR System
Catalogue number: 4376600
Description:
The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.
Features of the StepOnePlus™ Real-Time PCR System include:
- Advanced software and instrumentation for performing a wide array of genomic assay;
- Sensitive 4-color optical LED recording system;
- Intuitive and robust software perfect for both first-time and advanced users;
- Simple and flexible instrument set-up and usage.
New StepOne™ Software
The StepOne™ Software included with the StepOnePlus™ Real-Time PCR System runs on the Windows® XP operating system and provides instrument control, data collection, and data analysis capabilities (analysis software is also compatible with Windows® 7). This latest version includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in Real-Time PCR Data Markup Language (RDML) for compatibility with MIQE guidelines.
Rapid and Simple Assay Set-Up
This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR (Fig 1). The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOnePlus™ Real-Time PCR System also includes a quick-start setup so that you start a run immediately and enter plate information at a later time.
Sensitive LED-Based 4 Color Fluorescence Reading
The StepOnePlus™ Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM™/SYBR® Green, VIC®/JOE™, NED™/TAMRA™, and ROX™ dyes. This cost-effective, 4-color, 96-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.
Compatible with Many Genomic Analysis Techniques
Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus™ Real-Time PCR System and StepOne™ Software.
Specifications:
| Calibrated Dye: | FAM™, JOE™, NED, ROX™, SYBR® Green I, TAMRA™, VIC® |
|---|---|
| Capacity: | 1 x 96-well plate, 12 x 8-tube strips, 96 x 0.1 ml tubes |
| Detection Method: | Primer-Probe Detection, SYBR |
| Dimensions: | 24.6 cm/9.7 in.(W) x 42.7 cm/16.8 in.(D) x 51.2 cm/20.2 in.(H) |
| Dynamic Range: | Linear Dynamic Range greater than 9 log units (detection) |
| For Use With (Equipment): | StepOnePlus™ |
| Format: | 0.1 ml tubes, 8-tube strips, 96-well plate |
| Green Features: | Sustainable packaging |
| High Throughput Compatibility: | Multiplexing |
| Optics: | 4 emission filters, Blue LED excitation source, photodiode |
| Passive Reference Dye: | No ROX, ROX (Pre-mixed), ROX (Separate Tube) |
| Peak Block Ramp Rate: | 4.6°C⁄sec |
| Product Line: | StepOnePlus™ |
| Product Size: | 1 instrument |
| Reaction Speed: | Fast, Standard |
| Reaction Volume Range: | 10-30 µl (Standard curve experiments: 40 µl in standard mode is validated) |
| Run Time: | <2 hrs⁄run (Standard Mode), <40 min⁄run (Fast Mode) |
| Sample Ramp Rate: | Fast mode: ± 2.2°C⁄sec, Standard mode: ± 1.6°C⁄sec |
| Sensitivity: | 1 copy |
| Temperature Accuracy: | 0.25°C (35 to 95°C) of display temperature |
| Temperature Range (Metric): | 4-100°C |
| Temperature Uniformity: | 0.25°C (35 to 95°C) of setpoint/display temperature |
| Thermal Cycling System: | Peltier-Based System |
| Weight: | 24 kg (53 lbs.) |
The StepOnePlus™ System supports many Real-Time PCR applications, including the following:
- SNP Genotyping
- Gene Expression Analysis
- MicroRNA Expression
- Protein Expression
- Translocation Analysis
- Gene Detection
- Viral Load Analysis
The included StepOne™ Software supports a variety of analysis methods, including the following:
- Standard Curve (absolute quantitation)
- Relative Standard Curve
- Comparative Ct (ΔΔCt) (relative quantitation)
- Genotyping and Presence/Absence
- Melt Curve Analysis (as a standalone application)
- High Resolution Melting (as a standalone application)